PhD Candidate DNA Damage Repair in Cancer at Leiden University Medical Center

PhD Candidate DNA Damage Repair in Cancer

Vacancy number: D.19.NR.RD04

Category: research & education

area: research

appointment: fulltime   

Hours: 36 hours per week

Type of employment: duration education

duration: 4 years

education: Academic

Salary: max. € 3,020

Closing date: 22-02-2019

The position

The department of Human Genetics is looking for a PhD student who is intrigued to study mechanisms that prevent cancer development, has experience in fundamental cancer research and would like to be part of a young and dynamic team.

In short

  • You received your MSc in molecular biology or a related field;

  • You have hands-on experience in standard molecular biology techniques;

  • You have an organized and independent work style and you have excellent communication skills;

  • You are motivated to study BRCA1 function in the maintenance of genomic stability.

What you will do

As a PhD student you will conduct a research project studying the role of BRCA1 protein complexes in homologous recombination. For this, you will study complex formation via proteomic approaches and the genetic interactions of the different complexes via genome-wide CRISPR-Cas9 screens. Furthermore, you will be involved in mechanistic studies on the effect of BRCA1 cancer mutations on genomic stability and will study the clinical relevance of these findings.

The department

The Department of Human Genetics is a broadly oriented research department, led by Professor Silvère van der Maarel, focusing on the genetic aspects of a variety of diseases, such as cancer, and the translation into clinical relevance. The department consists of 22 research units, embedded in four overarching research themes. You will work in the recently established research group led by Dr. Sylvie Noordermeer, which is embedded in the research theme ‘Genome instability and cancer’ and has a clear focus on better understanding the fundamental mechanisms of DNA damage repair to improve clinical management of cancer patients. More specifically, the research in the Noordermeer lab governs the study of the protein BRCA1 and its regulation and function in multiple protein complexes involved in homologous recombination and genome stability maintenance. We complement our fundamental research in mammalian cell line models with studies on clinical data of BRCA1-mutated tumours to enable better risk prediction of the wide variety of BRCA1 mutations present in tumours. We have previously uncovered mechanisms involved in therapy resistance of BRCA1-deficient cells to PARPi (Noordermeer et al., Nature, 2018).

Career at the LUMC

The LUMC helps you with maintaining and developing your knowledge and expertise. We offer internal courses and in-service training to assist you with your personal development. We also offer services in terms of personal development, mobility and career advice.

The profile

You are an enthusiastic and driven early-career scientist who likes to work in a young and dynamic team, unravelling fundamental and mechanistic cancer research questions. You have hands-on experience in common molecular biology tools (molecular cloning, mammalian cell culture, Western blotting, fluorescent microscopy, flow cytometry, etc). Experience with CRISPR/Cas9-mediated genome editing is an advantage, but not a requirement. You are organized, accurate and able to work autonomously within a team. You have a flexible attitude, possess excellent communication skills and are fluent in written and spoken English.

More information

You will be employed on the basis of a 36-hour week. Appointment is for a maximum of four years (upon positive evaluation after year one), to be completed with a doctoral thesis. Your salary amounts to a maximum of € 2,357 gross per month in the first year, progressing to a maximum of € 3,020 in the fourth year (scale Pro, CLA UMC).

References will be requested.

Read more about the research group

Read more about the research theme: ‘Genome instability and cancer’

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